QBI 2020 Accepted Abstracts2019-11-03T22:59:29+00:00

Current List of Accepted Abstracts

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# 270

Glycine receptor copy numbers and packing density at spinal cord synapses revealed by quantitative dual-colour super-CLEM

Stephanie Maynard, Olivier Gemin, Philippe Rostaing, Antoine Triller, Christian G Specht
ENS,

# 272

Isotropic 3D molecular resolution in cells with MINFLUX nanoscopy

Francisco Balzarotti, Jasmin Pape, Klaus Gwosch, Stefan W. Hell
Research Institute for Molecular Pathology, Goettingen, DE

# 275

Quantitative Comparison of Single Particle Tracking Algorithms Across Different Signal and Noise Levels

Ye Lin, Sean B. Andersson
Boston University,

# 278

Quantifying the Robustness of Topological Defects in Orientation Fields from Bio-Images

Karl B. Hoffmann, Ivo Sbalzarini
Technische Universität Dresden, Dresden, Germany,

# 280

Filament Sensor – A tool for near real time analysis of stress fiber formation in stem cells

Lara Hauke, Benjamin Eltzner, Carina Wollnik, Stephan Huckemann, Florian Rehfeldt
Georg August Universität Göttingen, Göttingen, DE

# 282

Quantifying Localization Accuracy in Single-Molecule Super-Resolution Microscopy

Hesam Mazidi, Tianben Ding, Arye Nehorai, Matthew D Lew
Washington University in St. Louis,

# 283

Towards Bayesian based smart microscopy

Juliette Griffie, Paul Rolland, Daniel Sage, Robin Lang, Volkan Cevher, Suliana Manley
EPFL, Lausanne, CH

# 285

Untying the Gordian Knot: Spatiotemporal Single Particle Tracking using Point Cloud Reduction

Jorge Zepeda O, Logan D Bishop, Chayan Dutta, Suparna Sarkar-Banerjee, Christy Landes
Rice University,

# 288

PSF-broadening due to fluorescence emission: principle and implications on imaging

Jan Becker, Rainer Heintzmann
Leibniz Institute for Photonic Technology,

# 290

Single molecule localisation lighfield microscopy

Leila A Muresan
University of Cambridge, Cambridge, GB

# 291

Deep Learning approaches for reliable quantification of muti-omics cell imaging datasets to interrogate RNA and protein spatial and temporal subcellular interactions

Emmanuel Bouilhol, Macha Nikolski, Benjamin Dartigues, Hayssam Soueidan, Robyn Brackin, Anca savulescu, Musa Mhlanga
CNRS, Merignac, FR

# 294

Multivariate spectral analysis for identification of spectral features of peripheral nerves using Raman microspectroscopy

Takeo Minamikawa, Yoshiki Terao, Ryosuke Shiomi, Takeshi Yasui
Tokushima University,

# 295

Joint denoising deconvolution

Jerome Boulanger, Nick Barry, Suman Kumar Maji
MRC-LMB, Cambridge, US

# 296

Towards quantum enhanced quantitative superresolution microscopy

Dan Oron, Ron Tenne, Uri Rossman, Gur Lubin
Weizmann Institute of science,

# 297

Homogeneous multifocal excitation for high-throughput super-resolution imaging

Dora Mahecic, Davide Gambarotto, Kyle Douglass, Denis Fortun, Maeva Le Guennec, Virginie Hamel, Paul Guichard, Suliana Manley
Ecole Polytechnique Federale de Lausanne,

# 299

3D Multicolor Nanoscopy at 10,000 Cells a Day

Andrew E S Barentine, Yu Lin, Miao Liu, Phylicia Kidd, Leonhard Balduf, Michael Grace, Siyuan Wang, Joerg Bewersdorf, David Baddeley
Yale University,

# 301

Simple, compact and robust phase and fluorescence microscope for statistical analysis of cell cycle

Ondrej Mandula, Jean-Philippe Kleman, Francoise Lacroix, Cédric Allier, Daniel Fiole, Sophie Morales
CEA-Leti, Grenoble Cedex 9, FR

# 302

Phase Retrieval and Point Spread Function Design for Single Molecules Based on the Vectorial Diffraction Model

Boris Ferdman, Lucien Weiss, Elias Nehme, Yoav Shechtman
Technion – Israel Institute of Technology, Adi, IL

# 303

BioImage-IT

Sylvain Prigent, Charles Kervrann, Jean SALAMERO
Inria, Rennes, FR

# 306

3D tracking of endocytic and exocytic events using lattice light sheet microscopy

Cesar Augusto VALADES CRUZ, Ludovic LECONTE, Christian Wunder, Charles Kervrann, Ludger Johannes, Jean SALAMERO
Institut Curie, Paris, FR

# 307

Fluorescence Speckle Correlation Spectroscopy

Anirban Sarkar, Irène Wang, Jörg Enderlein, Jacques Derouard, Antoine Delon
Université Grenoble Alpes, , FR

# 309

Metal-Induced Energy Transfer

Jörg Enderlein
Georg August University, Göttingen, DE

# 310

Multimodal and multicolour microscopy to identify biomolecules in large-scale EM

Pascal de Boer, Aditi Srinivasa Raja, Jacob Hoogenboom, Ben Giepmans
University Medical Center Groningen,

# 311

Mechanical investigations of mEos4b blinking: longer single particle tracking with less track interruptions.

Siewert Hugelier, Elke De Zitter, Daniel Thédié, Viola Monkemöller, Joel Beaudouin, Virgile Adam, Martin Byrdin, Luc Vanmeervelt, Dominique Bourgeois, Peter Dedecker
KU Leuven, Heverlee, BE

# 312

A General Framework for Fitting an Arbitrary Model to SMLM Data

Yu-Le Wu, Markus Mund, Philipp Hoess, Aline Tschanz, Jonas Ries
EMBL, Heidelberg, DE

# 313

Toward Absolute Molecular Numbers in DNA-PAINT

Johannes Stein, Florian Stehr, Patrick Schueler, Philipp Blumhardt, Florian Schueder, Jonas Muecksch, Ralf Jungmann, Petra Schwille
Max-Planck-Institute of Biochemistry,

# 317

Stoichiometric quantification of spatially dense assemblies with qPAINT

Daniel J Nieves
University of Birmingham,

# 320

3D model-based restoration methods for 3D-SIM

Cong Tuan Son Van, Chrysanthe Preza, Hasti Shabani
University of Memphis,

# 322

Haste makes waste – Slow imaging increases resolution and labeling efficiencies in SMLM

Robin Diekmann, Maurice Kahnwald, Jonas Ries
EMBL,

# 324

An image-based machine learning approach as screening tool of pathological calcium oxalate crystallization inhibitors

Anna Kletzmayr, Jean-Christophe Leroux, Mattias E. Ivarsson
ETH Zurich,

# 325

Condensation of endoplasmic reticulum membrane domains

Konstantin S.A. Speckner, Lorenz Stadler, Matthias Weiss
University of Bayreuth, , DE

# 326

Simulation and implementation of digital micromirror devices (DMDs) for cost-effective structured illumination microscopy

Alice Sandmeyer, Mario Lachetta, Hauke Sandmeyer, Wolfgang Hübner, Thomas Huser, Marcel Müller
KU Leuven, Leuven, BE

# 329

Towards correlative multimodal nanospectroscopy

Iztok Urbancic, Falk Schneider, Erdinc Sezgin, Silvia Galiani, Christian Eggeling
Jozef Stefan Institute,

# 330

Conquering the tumour microenvironment: Using image analysis and spatial statistics to map the phenotypic heterogeneity of human cancers

Jack Robertson, Guillaume Potdevin, Brenda Beatriz Garcia Iglesias, Fargol Mazaheri, Maria Udriste, Stephanie Ling, Edmond Wong, Richard Goodwin, Hadassah Sade, Jaime Rodriguez Canales, Guenter Schmidt, Andrew Pierce
Definiens,

# 331

Organization and dynamics of Escherichia coli outer membrane proteins

Patrick George Inns, Nicholas Housden, Joanna Szczepaniak, Renata Kaminska, Colin Kleanthous
University of Oxford,

# 335

High-throughput multicolor 3D localization in live cells by depth-encoding imaging flow cytometry

Lucien Weiss, Yael Shalev Ezra, Sarah Goldberg, Boris Ferdman, Onit Alalouf, Yoav Shechtman
Technion, Israel Institute of Technology, Haifa, IL

# 336

Understanding and Modeling Inflammation Induced Immuno-Suppression in Tumors Using Multi-plex Immunofluorescence and Live Cell Microscopy

Stephen Lockett, Anne Gilmore, William Heinz, David Scheiblin, Sarah Flaherty, Noemi Kedei, Veena Somasundaram, Lisa Ridnour, Adelaide Wink, Caleb Kim, Valentin Magidson, Erina Kamiya, Jinqiu Chen, David Wink
National Cancer Institute,

# 337

Automated lens-free, fluorescent microscopy to study cell populations

Dorothee Kraemer, Cédric Allier, Lionel Hervé, Kiran Padmanabhan, Adlen Foudi, Sophie Morales
CEA-LETI,

# 339

Automating FLImP – macromolecular structure fingerprints for personalised cancer therapy

Daniel James Rolfe
Rutherford Appleton Laboratory,

# 341

Faster FLImP – Towards High throughput image acquisition and track selection

Ben Davis, Michail Vrettas, Michael Hirsch, Sarah Needham, Marisa Martin-Fernandez, Daniel James Rolfe
STFC,

# 342

Multi-parameter Single Molecule Spectroscopy Imaging Reveals Intra- and Inter- Molecular Conformations of HIV-1 Envelope on Native Viruses

Irene Carlon-Andres, Sergi Padilla-Parra
University of Oxford,

# 343

Towards multi-dimensional FLImP

Michail Vrettas, Daniel James Rolfe, Ben Davis
STFC,

# 344

Breaking New Ground in Live and Label-free Volumetric Imaging

Niall Hanrahan, Simon Lane, Raquel Munoz, Sumeet Mahajan
University of Southampton, Southampton, GB

# 345

User-friendly Building of Reconstruction Algorithms with GlobalBioIm

Laurène Donati, Emmanuel Soubies, Michael Unser
EPFL,

# 346

Automated, User-independent Correction of Artifacts in Fluorescence Correlation Spectroscopy Measurements using Convolutional Neural Networks

Alexander Seltmann, Christian Eggeling, Dominic Waithe
Friedrich-Schiller-University Jena,

# 347

SpineJ: A software tool for quantitative analysis of nanoscale spine morphology

Florian Levet, Jan Tonnesen, Valentin Nägerl, Jean-Baptiste Sibarita
Interdisciplinary Institute for NeuroScience, Bordeaux, FR

# 348

Developing microscope software with Python

Mick A Phillips, David Miguel Susano Pinto, Tiago Susano Pinto, Richard M Parton, Chris Weisiger, Andrew Jefferson, John W Sedat, Ilan Davis, Ian M Dobbie
University of Oxford,

# 349

Random Illumination Microscopy (RIM) : nanoscopy in living tissues with neither calibration nor adaptive optics

Thomas Mangeat, Simon Labouesse, awoke negash, Emmanuel Martin, Renaud Poincloux, Xiabo Wang, Magali Suzanne, Mathieu Pinot, Roland LE BORGNE, nicolas Sandeau, Kamal Belkebir, Hugues giovannini, Marc Allain, Jérome Idier, Anne Sentenac
CBI, Toulouse, FR

# 350

An extended quantitative model for super-resolution optical fluctuation imaging (SOFI)

Peter Dedecker, Wim Vandenberg, Sam Duwé, Marcel Leutenegger
KU Leuven, Leuven, BE

# 355

High density 3D localization of fluorescent molecules

Valentin Debarnot, Thomas Mangeat, Pierre Weiss
ITAV, CNRS, Toulouse, FR

# 357

Innovative buffer for long-lived fluorescence imaging for 2D and 3D dSTORM

Angelina Provost, corentin Rousset, Camille Fourneaux, Philippe Bouvet, Jean-Jacques Diaz, Catherine Ladavière, marie-therese charreyre, Arnaud Favier, Christophe Place, Karine MONIER
CRCL,

# 359

Single-Particle Diffusion Characterization by Deep Learning

Yoav Shechtman, Naor Granik, Yael Roichman, Lucien Weiss, Elias E Nehme, Eran Perlson
Technion, Israel Institute of Technology, Haifa, IL

# 360

DeepSTORM3D: Deep learning for dense 3D single molecule localization microscopy

Elias E Nehme, Daniel Freedman, Racheli Gordon, Boris Ferdman, Lucien Weiss, Onit Alalouf, Reut Orange, Tomer Michaeli, Yoav Shechtman
Technion – Israel Institute of Technology, Haifa, IL

# 362

Creating interactive, explorable simulations of cell migration at tissue scale using CPMjs

Inge M N Wortel, Johannes Textor
Radboud Institute for Molecular Life Sciences,

# 363

High-Throughput Structured Illumination Microscopy (HiT-SIM)

Robin Van den Eynde
KU Leuven,

# 364

Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI)

Robin Van den Eynde
KU Leuven,

# 365

Quantitative image-analysis of organoids with high-throughput digital holography microscopy

Thanh-an Pham, Nathalie Brandenberg, Sylke Hoehnel, Benjamin Rappaz, Michael Unser, Matthias Lütolf, Daniel Sage
EPFL, St-Sulpice, CH

# 366

KDML: a Knowledge-Driven Machine Learning for Analysing High Throughput Imaging Data

Heba Sailem, Jens Rittscher, Lucas Pelkmans
University of Oxford, Oxford, GB

# 367

Image-based nanoparticle characterization to aid drug design

Shoh Michael Asano, Katherine J. Hales
Pfizer,

# 368

Thermoregulation of immune cell dynamics

Stefan Wieser, Jaime Ortega Arroyo, Bernard Ciraulo, Ivan Company Garrido, Romain Quidant, Verena Ruprecht
ICFO – The Institute of Photonic Sciences, Castelldefels, ES

# 369

Spatiotemporal Resolution as an Information Theoretical Property of Stochastic Optical Localization Nanoscopy

Yi Sun
The City College of City University of New York, New York, US

# 370

Localization microscopy shows the 3D organization of LPS in the Escherichia coli outer-membrane

Sandip Kumar
University of Oxford,

# 371

FOCAL3D: A 3D clustering algorithm for single-molecule localization microscopy

Daniel Felipe Nino, Josh Milstein
University of Toronto, Mississauga, CA

# 372

An LPV approach to the estimation of time-varying Single Particle Tracking models

Boris I Godoy, Nicholas Vickers, Sean Andersson
Boston University, Boston, US

# 373

Measurement of the depth-dependence of point spread functions near the glass-water interface in high-numerical aperture microscopy

Petar N Petrov, William E. Moerner
Stanford University, Stanford, US

# 374

The dual approach to non-negative super-resolution

Bogdan Toader
University of Cambridge,

# 375

Model-based Imaging Biomarkers for Non-rectangular Regions of Interest

ShengLi Tzeng
National Sun Yat-sen University,

# 376

Multiplexed imaging of lung tissue using a macroscopic light sheet fluorescence microscope with uniform axial resolution

Arianna Gentile Polese, Gregory Seedorf, Leonardo Saunders, Dominik Stich, Douglas Parker Shepherd
Arizona State University,

# 377

Feature representations for the identification of mRNA localization patterns from smFISH data

Arthur Imbert, Edouard Bertrand, Florian Mueller, Thomas Edgar Walter
Mines ParisTech,

# 378

Axial stimulated emission depletion spectroscopy to study lipid dynamics

Aurélien Barbotin, Iztok Urbancic, Martin Booth, Christian Eggeling, Erdinc Sezgin
University of Oxford, Oxford, GB

# 379

Simultaneous multicolor single-molecule imaging using discrete PSF engineering

Wim Vandenberg, Marcel Leutenegger, Fabian Hertel, Bartosz Krajnik, Arno Bouwens, Robin Van den Eynde, Ezra Bruggeman, Thomas Schlichthärle, Alexander Auer, Ralf Jungmann, Peter Dedecker
KU Leuven, Leuven, BE

# 380

Separation of cell signalling responses based on a new model for photoswitching in FRET biosensors

Thijs Roebroek
KULeuven,

# 381

A 2-step algorithm for the estimation of time-varying Single Particle Tracking models

Boris I Godoy, Ye Lin, Sean Andersson
Boston University, Boston, US

# 382

Method of Synthetic Motion for Testing Single Particle Tracking Microscopes

Nicholas Vickers, Sean Andersson
Boston University, Quincy, US

# 383

Quantitative analysis of the structural organization and diffusion properties of a bacterial biofilm matrix

Valentin Dunsing, Tobias Irmscher, Stefanie Barbirz, Salvatore Chiantia
University of Potsdam, Berlin, DE

# 384

4Pi-SMLM and the 3D architecture of the Nuclear Pore Complex

Mark Bates
Max Planck Institute for Biophysical Chemistry, Goettingen, DE

# 385

Single-Cell / Single-Molecule microscopy on endogenous pulsed-labeled p53 dissects the regulation layers involved in its activation

Eugenia Cammarota, Alessia Loffreda, Matteo Mazzocca, Valeria Caiolfa, Moreno Zamai, Carlo Tacchetti, Davide Mazza
Ospedale San Raffaele,

# 386

SIMPLE: Structured illumination based point localization estimator with enhanced precision

Loïc Reymond, Stefan Wieser, Verena Ruprecht
ICFO – The Institute of Photonic Sciences,

# 387

Leukocyte mech(n)anobiology: super-resolution microscopy reveals actin nanoscale architecture at cell protrusions

Alessandra Cambi, Koen van den Dries
Radboud UMC, RIMLS, Nijmegen, NL

# 388

DeepFocus: a Near One-Shot Micro-Manager Auto-focus Plugin using Convolutional Neural Networks

Adrian Shajkofci, Michael Liebling
Idiap Research Institute, Martigny, CH

# 389

A comprehensive system calibration protocol for widefield fluorescence microscopy

Sreevidhya Ramakrishnan, Jerry Chao, Anish V Abraham, Sungyong You
Texas A&M University, , United States

# 390

Probabilistic overall reconstruction of membrane-associated molecular dynamics from partial observations in rod-shaped bacteria

Yunjiao LU, Charles Kervrann, Alain Trubuil, Pierre Hodara
INRA, Jouy-en-Josas, FR

# 391

Lipid Membrane Sensing and Remodeling during the immune synapse

Jorge Bernardino de la Serna, Esther A Garcia Gonzalez
Imperial College London,

# 395

Analysis of Nieman Pick C2 Protein in Regulation of Intracellular Membrane Transport by Combined and Correlative Fluorescent and Soft X-ray Microscopy

Alice Dupont Juhl, Daniel Wüstner
The University of Southern Denmark, Årslev, DK

# 396

Quantitative Phase Imaging

Alexander Jesacher
Medical University of Innsbruck,

# 397

Off-focus imaging exploits supercritical-angle fluorescence emission for precise SMLM

Alexander Jesacher, Philipp Zelger, Lisa Bodner, Lukas Velas, Gerhard Schütz
Medical University of Innsbruck,

# 398

Single-molecule approach to study mechanical control of B cell antigen recognition

Anna Teresa Bajur, Maria Iliopoulou, Kaltelyn Spillane
King’s College London,

# 400

Edge correction in density estimation from repeated point patterns in biological imaging

Jasmine Burguet, Philippe Andrey
INRA, Versailles, FR

# 402

Super-resolution Microscopy Elucidates Curvature Generation By Endocytic Clathrin Coats In Live Cells and Tissues

Comert Kural
Ohio State University,

# 404

Quantitative Analysis of Spatial-Temporal Imaging of Auxin to Cytokinin Ratio During Nodule Development in Soybean (Glycine max) by Two-Photon Induced Fluorescence

Ni Putu Dewi Nurmalasari, Paul Gaillard, Senthil Subramanian, Steve Smith
South Dakota School of Mines and Technology,

# 405

Investigating spatial relationships of host-associated microbes using correlative light and electron microscopy

Martin Thomas Jahn
GEOMAR Helmholtz Centre for Ocean Research,

# 409

Precise mapping of intracellular diffusion and drift from SPT data analysis

Antoine Salomon, Charles Kervrann
Inria Rennes, Rennes, FR

# 410

3D TIRF nanoscopy with sub-10 nm resolution

Sabrina Simoncelli, Alan Szalai, Brueno Siarray, Jeronimo Lukin, David Williamson, Nicolas Unsain, Damian Refojo, Alfredo Caceres, Mauricio Pilo-Pais, Guillermo Acuna, Dylan Owen, Fernando Stefani
King’s College London,

# 411

Thermophoretic trap for single amyloid fibril and protein aggregation studies

Tobias Thalheim
Peter Debye Institute for Soft Matter Physics, Leipzig University,

# 412

Comprehensive In-situ Profiling of Brain Cellular Alterations in the Injured Brain for Accelerating Drug Discovery

Badri Roysam
University of Houston,

# 413

Applications of single-molecule localisation microscopy to studying negative regulators of T cell activation

Dylan M Owen, Sabrina Simoncelli
University of Birmingham,

# 414

Multiconjugate Adaptive Optics for Microscopy

Karen M Hampson, Jiahe Cui, Matthew Wincott, Syed Asad Hussain, Kaustubh Banerjee, Pouya Rajaeipour, Hans Zappe, Caglar Ataman, Martin Booth
University of Oxford,

# 415

4Pi aberration correction in single molecule switching microscopy

Jingyu Wang, Martin Booth, Ian M Dobbie, Jacopo Antonello, Mick A Phillips, Richard M Parton, Chao He
University of Oxford,

# 416

A universal framework for sensorless adaptive optics

Qi Hu, Jingyu Wang, Matthew Wincott, Jacopo Antonello, Martin Booth
University of Oxford,

# 417

Implementation of Confocal Adaptive Optical Microscopy for Clinical Tissue Analysis

Jiahe Cui, Raphael Turcotte, Karen M Hampson, Martin Booth
University of Oxford,

# 418

Uniting Structured Illumination and Localization Microscopy (SIMFLUX)

Carlas Smith
TU-Delft,

# 419

Quantitative analysis of macropinocytosis in Dictyostelium captured with light-sheet microscopy

Josiah Lutton, Daniel Moore, Sharon Collier, Piotr Baniukiewicz, Till Bretschneider
University of Warwick,

# 420

Quantitative 3D super-resolution imaging of chromatin ‘blobs’

Lothar Schermelleh
University of Oxford,

# 421

Selective volumetric microcopy for single molecule imaging in living cells

Tommaso Galgani, Remi Galland, Virgile Viasnoff, Jean-Baptiste Sibarita, Bassam HAJJ
Institut Curie – CNRS, Paris, FR

# 422

Virtual Reality for Quantification of Multidimensional Single-Molecule Data

Thomas Blanc, Bassam HAJJ, Mohamed El Beheiry, Jean-Baptiste Masson
Institut Curie,

# 423

Superresolution Microscopy Images: What they tell us about protein clusters – and what they don’t

Gerhard J. Schütz
Vienna University of Technology, , Austria

# 424

Deciphering genuine transient pauses in super-localized trajectories of single-molecule tracers

Jaladhar Mahato, Arindam Chowdhury
Indian Institute of Technology Bombay,

# 426

3D-SRRF: Real-Time Live-Cell-Compatible Volumetric Super-Resolution Microscopy

Romain F Laine, Tommaso Galgani, Bassam HAJJ, Ricardo Henriques
UCL,

# 427

Calibration of a Remote Focusing Microscope Using Deflectometry

Syed Asad Hussain, Karen M Hampson, Matthew Wincott, Jacopo Antonello, Martin Booth
University of Oxford,

# 430

Quantification of DNA by Mass Photometry

Yiwen Li, Katharina Haeussermann, Weston Struwe, Philipp Kukura
University of Oxford,

# 431

Site-selective visualization and tracking of DNA methylation in fixed cells

Krystian Ubych, Paco Fernandez-Trillo, Clare Davies, Robert Neely
University of Birmingham,

# 433

Ultrashort dual colour laser at 1.55 and 1.88 μm for the detection of tumour through Raman imaging

Syed Asad Hussain
University of Oxford,

# 435

ConCoR: A software pipeline for high-throughput image analysis of time-dependent colocalization data

Greg Bass, Isabelle Glauser, Jenny Chia, Anne Verhagen, Steve Dower, Milica Ng
CSL Innovation,

# 437

Models of single molecule dynamics from single photon detections: a Bayesian nonparametric approach

Steve Pressé
Arizona State University, Tempe, US

# 438

FCS and anomalous diffusion model as tools to monitor molecular interactions in living cells

Alessandro Furlan, Aymeric Leray, Melanie Henry, Mariano Gonzalez Pisfil, Dorian champelovier, Thorsten Wohland, Corentin Le Nezet, Olivier Benbsude, Bernard Vandenbunder, Gabriel Bidaux, Laurent HELIOT
Univ-Lille,

# 439

CLIJ: GPU-accelerated real-time image analysis is the key to smart microscopy

Robert Haase, Daniela Vorkel, Akanksha Jain, Nicola Maghelli, Pavel Tomancak, Eugene W. Myers
Max Planck Institute for Molecular Cell Biology and Genetics,

# 440

Toward SMLM acquisitions assisted by real-time quality control

Sébastien Mailfert, Nicolas Bertaux, Didier Marguet
Centre d’Immunologie de Marseille Luminy, Marseille cedex 09, FR

# 441

Analysis of relative positions in 3D PALM and dSTORM provides high-resolution information on ordered biological complexes

Alistair Curd, Ruth Hughes, Michelle Peckham
University of Leeds,

# 442

Dynamic contrast for micro- and macro-scale multiplexed fluorescence imaging against autofluorescence and ambient light

Raja CHOUKET, Ludovic Jullien, Thomas le Saux, Ruikang Zhang, Alison Tebo, Agathe Espagne, Arnaud Gautier, Marie Aude Plamont, Zsolt Kelemen, Jean-Denis Faure, Vincent Croquette, Lionel Gissot
Ecole Normale Superieure, , France

# 443

Statins – not just about cholesterol reduction

Dilip Shrestha
University of Oxford,

# 444

Nanoscale cellular dynamics with super-resolution spectroscopy

Erdinc Sezgin, Christian Eggeling
University of Oxford, , GB

# 445

Quantification of bone tissue heterogeneity and cell distribution patterns from digital histology: application to osteosarcoma

Anthony Mancini, Anne Gomez-Brouchet, Michel quintard, Sylvie Lorthois, Pascal Swider, Pauline Assemat
IMFT,

# 446

Optimization of experimental and acquisition parameters for improved SOFI super-resolution microscopy imaging

Dario Cevoli
Université de Lille,

# 448

Epidermal growth factor receptor localization and dynamics inside nanodomains across different cell types

Thuy Thi HOANG, Nicolas Olivier, Hélène Lazareth, Pierre-Louis Tharaux, Cedric Bouzigues, Antigoni Alexandrou
école polytechnique,

# 450

Measuring diffusion dynamics at high photon-count rates with FCS and STED-FCS

Falk Schneider, M. Julia Roberti, Erdinc Sezgin, Christian Eggeling, Iztok Urbančič
University of Oxford, Oxford, GB

# 452

ALFRED: user-friendly approach to obtain length and curvature and its application to microtubule networks in nerve cells

Beatriz Costa-Gomes, Nuno Nobre, André Voelzmann, Matthias Heil, Andreas Prokop
University of Manchester,

# 453

Cryo-CARE Facilitates Sub-Tomogram Averaging in Cryo Transmission Electron Microscopy

Tim-Oliver Buchholz, Mareike Jordan, Gaia Pigino, Florian Jug
MPI-CBG/CSBD,

# 454

Cluster analysis of SMLM data

Dylan M Owen
University of Birmingham,

# 455

3D Model Building Pipeline for Light-Sheet Imaging of Hippocampal Neurons for Simulation and Analysis of Calcium Dynamics

James Barnard Wilsenach, Charlotte M. Deane, Gesine Reinert
University of Oxford,

# 457

Extending molecular counting by photon statistics

Johan Hummert, Klaus Yserentant, Wioleta Chmielewicz, Dirk-Peter Herten
Heidelberg University, Berlin, DE

# 458

Cell segmentation and tracking for mathematical modelling of cell motility

Rituparna Sarkar, Pascal Bochet, Maria Manich, Aleix Boquet-Pujadas, Nancy Gullien, Elisabeth Labruyere, Jean-Christophe Olivo-Marin
Institute Pasteur,

# 460

Quantitating light sheet microscopy

James Manton, Nick Barry
MRC Laboratory of Molecular Biology,

# 461

Cluster Analysis of Localization Microscopy Data with Machine Learning

David Williamson, Garth Burn, Sabrina Simoncelli, Ruby Peters, Juliette Griffie, Daniel Davis, Dylan Owen
King’s College London,

# 462

Investigating membrane proteins interactions and membrane mimetic systems using mass photometry

Anna Olerinyova, Philipp Kukura, Weston Struwe, Adar Sonn-Segev
University of Oxford,

# 463

Quantifying binding affinities and kinetics of biomolecular complexes with mass photometry

Fabian Soltermann, Weston Struwe, Philipp Kukura
Oxford University,

# 465

Adaptive optics in bioimaging for improved quantitative exploitation of microscopy images

Audrius Jasaitis, Cynthia Veilly, Fabrice Harms, Xavier Levecq
Imagine Optic,

# 466

Quantitative imaging of intracellular receptors in cardiac myocytes using DNA-PAINT

Anna Meletiou, Christian Soeller
University of Exeter,

# 467

Characterization of Flat-Fielding Systems for Super-Resolution Microscopy

Khalid Ibrahim, Dora Mahecic, Suliana Manley
EPFL,

# 468

Quantitative imaging of sparse targets with DNA-PAINT

Alexander H Clowsley, Christian Soeller
The University of Exeter,

# 469

Identifying analysis algorithm induced image artefacts in localisation microscopy

Richard John Marsh, Ishan Costello, Susan Cox
King’s College London, London, GB

# 470

Bayesian Grouping of Localizations: Sub-Nanometer Precision, Counting and Resolution Doubling

Keith A Lidke
University of New Mexico, Albuquerque, US

# 471

Towards a molecular mechanism of dynamin polymerization with mass photometry

Manish Singh Kushwah, Philipp Kukura
University of Oxford,

# 473

Probabilistic Noise2Void: Unsupervised Content-Aware Denoising

Alexander Krull, Tomas Vicar, Mangal Prakash, Manan Lalit, Florian Jug, Pavel Tomancak
MPI-CBG/CSBD,

# 475

Leveraging Self-Supervised Denoising for Image Segmentation

Mangal Prakash, Tim-Oliver Buchholz, Manan Lalit, Pavel Tomancak, Florian Jug, Alexander Krull
MPI-CBG/CSBD,

# 476

Bootstrapping Unsupervised Image Denoising with Parametric Noise Models

Mangal Prakash, Manan Lalit, Pavel Tomancak, Alexander Krull, Florian Jug
MPI-CBG/CSBD,

# 477

Quantitative Imaging Using Super-Resolution Optical Fluctuation Imaging

Kristin S. Grussmayer, Adrien Descloux, Aleksandra Radenovic, Theo Lasser, Tomas Lukes, Marcel Leutenegger, Stefan Geissbuehler
EPFL,

# 479

Biolapse Toolbox

Léo Lebrat, Valentin Debarnot, Thomas Mangeat
CSIRO Health and Biosecurity Flagship: The Australian e-Health Research Centre Brisbane, QLD, Australia,

# 480

Single-molecule and super-resolution investigation of chemokine receptor behaviour within the T cell immunological synapse

James Felce, Michael Dustin
University of Oxford,

# 481

Single Molecule Localization Microscopy using time modulated illumination

Pierre Jouchet, Clement Cabriel, Nicolas Bourg, Marion Bardou, Christian Poüs, Emmanuel Fort, Sandrine Leveque-Fort
CNRS, Orsay Cedex, FR

# 482

PhenoPlot-Web for visualising microscopy data

Andrea Chatrian, Jens Rittscher, Heba Sailem
University of Oxford, Oxford, GB

# 483

ICYTOMINE: A User-Friendly Tool for Reproducible Analysis of Gigapixel Images

Daniel Felipe González Obando, Diana Mandache, Jean-Christophe Olivo-Marin, Vannary Meas-Yedid
Institut Pasteur,

# 484

Multiplex Immune Cell Profiling to Gain Insights into Progression in Early Cancer

Jens Rittscher, Natalia Garcia Martin
University of Oxford,

# 485

Leveraging Automated Blood Cell Morphology for Myelodysplastic Syndrome Diagnosis and Prognosis Prediction

José Guilherme de Almeida, George Vassiliou, Moritz Gerstung
European Bioinformatics Institute,

# 486

Generalized Statistical Object Distance Analysis (GSODA) For Object Based Colocalization In Quantitative Microscopy

Suvadip Mukherjee, Thibault Lagache, Daniel Gonzalez-Obando, Vannary Meas-Yedid, Lydia A Danglot, Jean-Christophe Olivo-Marin
Institut Pasteur,

# 487

Label-free live-cell imaging at 150 nm and 100 Hz by ROCS-Microscopy

Alexander Rohrbach
University of Freiburg,

# 488

Full field interferometry for characterizing and counting biotic nanoparticles

Samer Alhaddad, Yasmina Fedala, Ignacio Izeddin, Claude Boccara, Martine Boccara
ESPCI, PSL University, CNRS,

# 489

Statistical modeling of spatial interactions in biological patterns, with application to plant nuclear organization

Javier Arpon, Kaori Sakai, Valérie Gaudin, Philippe Andrey
INRA, Versailles, FR

# 490

Quantitative mapping of fluorescently tagged ribosomes throughout the cell cycle of S. cerevisiae using FCS-calibrated imaging

Madlen Luckner, Gabriele Schreiber, Edda Klipp, Salvatore Chiantia
University of Potsdam,

# 491

Versatile uniform illumination method toward quantitative single molecule localization microscopy

Adrien Mau, Nicolas Bourg, Sandrine Leveque-Fort
Ismo / Abbelight,

# 492

Quantitative analysis of EGF receptor dynamics, confinement and interaction with the cytoskeleton using nanoparticle labelling, single-molecule tracking and hydrodynamic force application

Chao Yu, Maximilian Richly, Cedric Bouzigues, Antigoni Alexandrou
Ecole polytechnique,

# 493

Molecular counting with calibrated fluorescent labelling and photobleaching step analysis

Klaus Yserentant, Johan Hummert, Felix Braun, Wioleta Chmielewicz, Dirk-Peter Herten
Heidelberg University, Heidelberg, DE

# 494

Multiplex Single Molecule Pull-down (SiMPull) measurements of EGFR site-specific phoshporylation

Diane Lidke
University of New Mexico, Albuquerque, US

# 495

Machine learning and airy-light-sheet microscopy reveals morphometric indicators in organoids for informed anti-cancer drug discovery

Craig Terence Russell
National Physical Laboratory,

# 499

Computational analysis of filament polymerization dynamics in cytoskeletal networks

Paulo Caldas, Christoph Sommer, Philipp Radler, Martin Loose
Institute of Science and Technology Austria,

# 503

Novel particle tracking approach with CNN-based candidate selection and two-step linking by Bayesian network

Mariia Dmitrieva, Jens Rittscher
University of Oxford,

# 505

Measuring differential diffusion rates of distinct protein states by sptPALM imaging in live microbial cells

Bartosz Turkowyd
Max Planck Institute for terrestrial Microbiology,

# 506

Metadata and Performance Tracking for Fluorescent Microscopes

Mathias Hammer, Alex Rigano, Farzin Farzam, Andrew Shanaj, Colton Horman, Carlas Sierd Smith, Russell Ulbrich, Caterina Strambio de Castilla, David Grunwald, Maximiliaan Huisman
UMass Medical School, WORCESTER, US

# 507

Metadata and Performance Tracking for Fluorescent Microscopes III – MetaMax

Maximiliaan Huisman, Carlas Sierd Smith, Mathias Hammer, Russell Ulbrich, David Grunwald
UMass Medical School, WORCESTER, US

# 508

Metadata and Performance Tracking for Fluorescent Microscopes I – Metadata

David Grunwald, Mathias Hammer, Alex Rigano, Maximiliaan Huisman, Farzin Farzam
Umass Medical School, Worceser, US

# 509

Metadata and Performance Tracking for Fluorescent Microscopes II – Optics

David Grunwald, Mathias Hammer, Maximiliaan Huisman, Farzin Farzam
Umass Medical School, Worceser, US

# 510

Novel intensity-based approach to characterize astrocytic calcium signals

Andre Zeug, Franziska Müller, Evgeni Ponimaskin, Gebhard Stopper
Hannover Medical School,

# 512

Towards Quantitative 3D Super-Resolution Correlative Light-Electron Microscopy

Jonas Euchner, Klaus Yserentant, Dirk-Peter Herten
Heidelberg University,

# 515

Pixelwise H-score: a novel digital image analysis-based metric to quantify membrane biomarker expression from IHC images

Sripad Ram
Pfizer, Inc., , United States

# 516

Functional summary statistics for Poisson processes on bounded convex three dimensional shapes

Scott Ward, Edward Cohen, Niall Adams
Imperial College London, London, GB

# 517

Deep learning based Phase Microscopy with Uncertainty Quantification

Lei Tian, Yujia Xue, Shiyi Cheng, Yunzhe Li
Boston University,

# 518

Using machine learning to design intelligent computational microscopes

Roarke Horstmeyer, Amey Chaware, Colin Cooke, Kanghyun Kim, Pavan Konda
Duke University,

# 520

Quantitative analyses of T cell signaling

Christoph Wuelfing
University of Bristol,

# 522

3Dscript: animating 3D/4D microscopy data using a naturallanguage- based syntax.

Ralph Palmisano
Friedrich-Alexander University Erlangen-Nürnberg,

# 524

ZOLA-3D: a software for optimal 3D SMLM with arbitrary PSF

benoit lelandais, MICKAEL LELEK, Jonas Ries, christophe Zimmer
institut pasteur, paris, FR

# 526

QuantEv: A Quantitative Approach for Analyzing the Spatio-Temporal Distribution of 3D Events in Fluorescence Microscopy

Thierry Pecot, Zengzhen Liu, Jerome Boulanger, Jean SALAMERO, Charles Kervrann
Medical University of South Carolina,

# 527

Inferring correlation in inhomogeneous and multitype point patterns – a review and new contributions

Rasmus Plenge Waagepetersen
Aalborg University,

# 528

Towards quantitative correlative microscopy

Lucy M Collinson
Francis Crick Institute,

# 529

Quantitative super-resolution imaging with DNA-PAINT

Christian Soeller
University of Exeter,

# 530

Machine Learning for Computational Phenotyping: how to overcome the need for massive image annotation

Thomas Edgar Walter
Mines ParisTech, Paris, FR

# 531

Label-free intracellular dynamics studies using quantitative phase imaging with a high definition wavefront sensor

Roman Zinchuk, Benoit Wattellier, Antoine Federici, Julien Savatier, Serge Monneret
Aix-Marseille Univ, CNRS, Centrale Marseille, Institut Fresnel,

# 533

Predicting Patient Outcome from Immunohistochemistry Images by Quantifying Spatial Distribution of Immune Cells

Morgan Oneka, Jenny Lazarus, Souptik Barua, Arvind Rao, Timothy Frankel
University of Michigan,

# 535

Spatial Statistics Workshop

Edward Cohen
Imperial College London, London, GB

# 536

SMLM flight simulator (WORKSHOP)

Juliette Griffie, Daniel Sage
EPFL, Lausanne, CH

# 538

STATISTICAL AND COMPUTATIONAL METHODS FOR INTRACELLULAR TRAJECTORY ANALYSIS IN FLUORESCENCE MICROSCOPY

Charles Kervrann
Inria, Rennes, FR

# 569

Effect of pixelation on the parameter estimation of single molecule trajectories

Milad Rafiee Vahid, Bernard Hanzon, Raimund J Ober
Texas A&M University, College Station, US

# 571

Implementation of multiplexing and image analysis to support drug discovery and development

Shanon Seger
Roche,

# 572

3D parallelized RESOLFT optical nanoscopy

Ilaria Testa
KTH, SciLifeLab,

# 576

A BOLT-ON SINGLE-OBJECTIVE LIGHT-SHEET DESIGN WITH UNCOMPROMISED NUMERICAL APERTURE

Alfred Millett-Sikking, Andrew York
Calico Labs,

# 577

Imaging the nanoscale structure of endocytosis with correlative super-resolution light and electron microscopy.

Justin W Taraska, Kem Sochacki
National Institutes of Health,

# 579

Mesoscale imaging with the Mesolens

Gail McConnell
University of Strathclyde,

# 584

Multiplex imaging of lung tumours with CODEX technology

Marta Trüb, Petra Herzig, Nicole Kirchhammer, Franziska Werner, Kirsten Mertz, Viktor Kölzer, Abhishek Kashyap, Alfred Zippelius
University of Basel / University Hospital Basel,

# 585

Quantitative Multicolor Super-Resolution Microscopy

Jean-Baptiste Sibarita
Interdisciplinary Institute for NeuroScience, , France

# 586

Bayesian Inference of the Spatial Distribution of Proteins from Three-Cube Förster Resonance Energy Transfer Data

Jan-Otto Hooghoudt, Rasmus Plenge Waagepetersen
Aalborg University,

# 589

Quantitative super-resolution imaging in biology

Melike Lakadamyali
University of Pennsylvania,

# 590

Live-cell correlative microscopy of single organelles

Nalan Liv
University Medical Center Utrecht,,

# 592

Counting by Photon Statistics

Johan Hummert, Klaus Yserentant, Wioleta Chmielewicz, Dirk-Peter Herten
Heidelberg University, Berlin, DE